Journal: Signal Transduction and Targeted Therapy

Article Title: DEAD-box helicase 17 (DDX17) protects cardiac function by promoting mitochondrial homeostasis in heart failure

doi: 10.1038/s41392-024-01831-2

Figure Lengend Snippet: Cardiomyocyte-specific Ddx17 knockout leads to reduced cardiac function and exacerbates Dox-induced heart failure in mice. a , b Western blot and the average data of DDX17 in isolated NMVMs of control (Con) and Ddx17- cKO mice ( n = 6). c Western blot of DDX17 levels in heart, skeletal muscle, liver and kidney of Con and Ddx17 -cKO mice ( n = 3). d Mice were injected intraperitoneally with saline or 7.5 mg/kg Dox 3 times every other day. Survival curve of the mice in the four groups ( n = 18). e Body weight of mice in the control + saline (Con + Saline), Ddx17- cKO + saline ( Ddx17- cKO + Saline), control + doxorubicin (Con + Dox) and Ddx17- cKO + doxorubicin ( Ddx17- cKO + Dox) groups ( n = 5). f Representative images of the hearts from the mice in Con + Saline, Ddx17- cKO + Saline, Con + Dox, and Ddx17- cKO + Dox groups, the scale bar represents 2 mm. g Representative H&E-stained heart sections from mice in the four groups ( n = 5); scale bar, 50 μm. h , i Representative images of Sirius Red-stained hearts from mice in the four groups and semiquantitative analysis of the fibrosis area ratio ( n = 5); scale bar, 200 μm. j – l Representative images of echocardiography of mouse hearts and the average data of cardiac function of left ventricular EF ( k ) and FS ( l ) in the four groups; n = 10 for each group. m TUNEL staining quantification results of myocardial tissue in the four groups; n = 5 for each group. n , o Mouse serum LDH ( n ) and CK-MB ( o ) levels in the four groups ( n = 6). * P < 0.05, ** P < 0.01, and *** P < 0.001

Article Snippet: TUNEL staining (Cat. No. 4810-30-CK, R&D System, USA) of heart sections: TUNEL staining of heart sections was performed according to previously reported methods with modifications.

Techniques: Knock-Out, Western Blot, Isolation, Control, Injection, Saline, Staining, TUNEL Assay

Journal: Signal Transduction and Targeted Therapy

Article Title: DEAD-box helicase 17 (DDX17) protects cardiac function by promoting mitochondrial homeostasis in heart failure

doi: 10.1038/s41392-024-01831-2

Figure Lengend Snippet: Overexpression of Ddx17 in cardiomyocytes attenuates myocardial injury and improves cardiac function under pathological conditions. a Western blot of DDX17 protein expression from control (Con) and two cardiac-specific Ddx17 -overexpressing mouse lines ( Ddx17 -Tg-H and Ddx17 -Tg) ( n = 5). b DDX17 expression in heart, skeletal muscle, liver and kidney of control (Con) and Ddx17 -Tg mice ( n = 3). c Doxorubicin (7.5 mg/kg) or an equivalent volume of saline was administered to mice by intraperitoneal injection every other day for a total of three injections in the control (Con) and Ddx17 -Tg mice. Survival curves of mice in the control + saline (Con + Saline), Ddx17 -transgene + saline ( Ddx17 -Tg + Saline), control + doxorubicin (Con + Dox), and Ddx17 -transgene + doxorubicin ( Ddx17 -Tg + Dox) groups ( n = 18). d Body weight of the mice in the Con + Saline, Ddx17 -Tg + Saline, Con + Dox, and Ddx17 -Tg + Dox groups ( n = 6). e – g Representative images of echocardiography of mouse heart and the average data of cardiac function of left ventricle EF ( f ) and FS ( g ) in the four groups ( n = 11). h , i Representative images of heart morphologies and H&E-stained heart sections in the four groups ( n = 6). j – k Representative images of Sirius Red-stained mouse heart sections and quantification of myocardial fibrosis area ratio in the four groups ( n = 5); scale bar, 200 μm. l TUNEL staining of myocardial tissue in Con + Saline, Ddx17 -Tg + Saline, Con + Dox, and Ddx17 -Tg + Dox, ( n = 5); scale bar, 50 μm. m Quantification of TUNEL staining of myocardial tissue in the four groups ( n = 5). * P < 0.05, ** P < 0.01, and *** P < 0.001

Article Snippet: TUNEL staining (Cat. No. 4810-30-CK, R&D System, USA) of heart sections: TUNEL staining of heart sections was performed according to previously reported methods with modifications.

Techniques: Over Expression, Western Blot, Expressing, Control, Saline, Injection, Staining, TUNEL Assay

Journal: Frontiers in Immunology

Article Title: Adipose-derived stem cell exosomes suppress NLRP3-mediated neuronal pyroptosis to attenuate seizures in a kainic acid-induced temporal lobe epilepsy model

doi: 10.3389/fimmu.2025.1691814

Figure Lengend Snippet: Effects of ADSC-Exos on KA-induced hippocampal neuron damage. (A) Representative Nissl-stained images (200×) showing ADSC-Exos attenuated KA-induced neuron loss in hippocampal CA1 and CA3 regions. (B) Representative TUNEL-stained images (200×) showing ADSC-Exos alleviated KA-induced apoptosis in CA1 and CA3. (C–F) Quantitative analyses of (C) CA1 Nissl+, (D) CA3 Nissl+, (E) CA1 TUNEL+, and (F) CA3 TUNEL+ cells. Individual data points represent values from each mouse (n=5 per group). Data are expressed as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (one-way ANOVA with Tukey’s post hoc test).

Article Snippet: The assay was performed according to the manufacturer’s protocol (TUNEL Apoptosis Detection Kit, R&D Systems, Switzerland,CatNo.4810-30-CK).

Techniques: Staining, TUNEL Assay